Review



vegf promoter driven luciferase reporter plasmid  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Addgene inc vegf promoter driven luciferase reporter plasmid
    FIGURE 5 Araliadiol enhances hair growth-promoting signals and anagen phase markers in human hair follicle cells. (A) HHFSCs were treated with different concentrations of araliadiol (0–1.2 μg/mL) for 48 h. Protein levels of hair follicle stem cell-specific markers (K15 and K19), anagen phase markers (CD34 and p63α), and hair growth-promoting proteins <t>(VEGF</t> and ANGPTL4) were analyzed by Western blotting, with β-Actin serving as a loading control. (B) HDPCs were stimulated with indicated concentrations of araliadiol (0–2.5 μg/mL) for 48 h. Protein levels of hair growth-promoting proteins (FGF2, FGF7, FGF10, VEGF, IGF-1, and NOG) were analyzed by Western blotting, with β-Actin serving as a loading control. The protein levels in all blots were quantified using ImageJ software version 1.53t. K15, cytokeratin 15; K19, cytokeratin 19; CD34, cluster of differentiation 34; p63α, tumor protein 63 alpha; VEGF, vascular endothelial growth factor; ANGPTL4, angiopoietin like 4; FGF2, fibroblast growth factor 2; FGF7, fibroblast growth factor 7; FGF10, fibroblast growth factor 10; IGF-1, insulin-like growth factor 1; NOG, noggin.
    Vegf Promoter Driven Luciferase Reporter Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vegf+promoter+driven+luciferase+reporter+plasmid/pGL4%2E10-VEGFprom(-1000+to+-1)+(Plasmid+%2366128)/pm39691387-101-25-34
    Average 93 stars, based on 9 article reviews
    vegf promoter driven luciferase reporter plasmid - by Bioz Stars, 2026-11
    93/100 stars

    Images

    1) Product Images from "In vitro hair growth-promoting effects of araliadiol via the p38/PPAR-γ signaling pathway in human hair follicle stem cells and dermal papilla cells."

    Article Title: In vitro hair growth-promoting effects of araliadiol via the p38/PPAR-γ signaling pathway in human hair follicle stem cells and dermal papilla cells.

    Journal: Frontiers in pharmacology

    doi: 10.3389/fphar.2024.1482898

    FIGURE 5 Araliadiol enhances hair growth-promoting signals and anagen phase markers in human hair follicle cells. (A) HHFSCs were treated with different concentrations of araliadiol (0–1.2 μg/mL) for 48 h. Protein levels of hair follicle stem cell-specific markers (K15 and K19), anagen phase markers (CD34 and p63α), and hair growth-promoting proteins (VEGF and ANGPTL4) were analyzed by Western blotting, with β-Actin serving as a loading control. (B) HDPCs were stimulated with indicated concentrations of araliadiol (0–2.5 μg/mL) for 48 h. Protein levels of hair growth-promoting proteins (FGF2, FGF7, FGF10, VEGF, IGF-1, and NOG) were analyzed by Western blotting, with β-Actin serving as a loading control. The protein levels in all blots were quantified using ImageJ software version 1.53t. K15, cytokeratin 15; K19, cytokeratin 19; CD34, cluster of differentiation 34; p63α, tumor protein 63 alpha; VEGF, vascular endothelial growth factor; ANGPTL4, angiopoietin like 4; FGF2, fibroblast growth factor 2; FGF7, fibroblast growth factor 7; FGF10, fibroblast growth factor 10; IGF-1, insulin-like growth factor 1; NOG, noggin.
    Figure Legend Snippet: FIGURE 5 Araliadiol enhances hair growth-promoting signals and anagen phase markers in human hair follicle cells. (A) HHFSCs were treated with different concentrations of araliadiol (0–1.2 μg/mL) for 48 h. Protein levels of hair follicle stem cell-specific markers (K15 and K19), anagen phase markers (CD34 and p63α), and hair growth-promoting proteins (VEGF and ANGPTL4) were analyzed by Western blotting, with β-Actin serving as a loading control. (B) HDPCs were stimulated with indicated concentrations of araliadiol (0–2.5 μg/mL) for 48 h. Protein levels of hair growth-promoting proteins (FGF2, FGF7, FGF10, VEGF, IGF-1, and NOG) were analyzed by Western blotting, with β-Actin serving as a loading control. The protein levels in all blots were quantified using ImageJ software version 1.53t. K15, cytokeratin 15; K19, cytokeratin 19; CD34, cluster of differentiation 34; p63α, tumor protein 63 alpha; VEGF, vascular endothelial growth factor; ANGPTL4, angiopoietin like 4; FGF2, fibroblast growth factor 2; FGF7, fibroblast growth factor 7; FGF10, fibroblast growth factor 10; IGF-1, insulin-like growth factor 1; NOG, noggin.

    Techniques Used: Western Blot, Control, Software

    FIGURE 8 Araliadiol elevates hair growth-promoting effects in human hair follicle cells by upregulating the PPAR-γ signaling pathway. (A) Protein levels of PPAR-γ and hair growth-inductive proteins (K15, VEGF, and ANGPTL4) were assessed after treatment with araliadiol (1.2 μg/mL) with or without GW9662 (20 μM) in HHFSCs for 48 h. (B) Protein levels of PPAR-γ and hair growth-inductive proteins (FGF7, VEGF, and NOG) were assessed after treatment with araliadiol (2.5 μg/mL) with or without GW9662 (20 μM) in HDPCs for 48 h. Protein levels in all blots were quantified using ImageJ software version 1.53t.
    Figure Legend Snippet: FIGURE 8 Araliadiol elevates hair growth-promoting effects in human hair follicle cells by upregulating the PPAR-γ signaling pathway. (A) Protein levels of PPAR-γ and hair growth-inductive proteins (K15, VEGF, and ANGPTL4) were assessed after treatment with araliadiol (1.2 μg/mL) with or without GW9662 (20 μM) in HHFSCs for 48 h. (B) Protein levels of PPAR-γ and hair growth-inductive proteins (FGF7, VEGF, and NOG) were assessed after treatment with araliadiol (2.5 μg/mL) with or without GW9662 (20 μM) in HDPCs for 48 h. Protein levels in all blots were quantified using ImageJ software version 1.53t.

    Techniques Used: Software

    FIGURE 10 Araliadiol enhances VEGF secretion via the p38/PPAR-γ signaling pathway in human hair follicle cells. (A, B) Human hair follicle cells were treated with araliadiol (0–2.5 μg/mL) for 48 h, either with or without SB202190 (20 μM) or GW9662 (20 μM). VEGF-A secretion levels were quantified using an ELISA on conditioned medium from the treated cells. Minoxidil (2 μg/mL) was included as a positive control. Results are presented as the mean ± SD of three independent experiments and analyzed using a one-way analysis of variance followed by Tukey’s test. ***p < 0.001 compared with the vehicle-treated group. ##p < 0.01; ###p < 0.001 compared with the araliadiol-treated group. ELISA, enzyme-linked immunosorbent assay.
    Figure Legend Snippet: FIGURE 10 Araliadiol enhances VEGF secretion via the p38/PPAR-γ signaling pathway in human hair follicle cells. (A, B) Human hair follicle cells were treated with araliadiol (0–2.5 μg/mL) for 48 h, either with or without SB202190 (20 μM) or GW9662 (20 μM). VEGF-A secretion levels were quantified using an ELISA on conditioned medium from the treated cells. Minoxidil (2 μg/mL) was included as a positive control. Results are presented as the mean ± SD of three independent experiments and analyzed using a one-way analysis of variance followed by Tukey’s test. ***p < 0.001 compared with the vehicle-treated group. ##p < 0.01; ###p < 0.001 compared with the araliadiol-treated group. ELISA, enzyme-linked immunosorbent assay.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Positive Control

    Related Articles

    Plasmid Preparation:

    Article Title: In vitro hair growth-promoting effects of araliadiol via the p38/PPAR-γ signaling pathway in human hair follicle stem cells and dermal papilla cells.
    Article Snippet: .. The pSV-βgalactosidase plasmid (#E1081) was sourced from Promega, whereas the Gli response element-driven luciferase reporter plasmid (#113712), TCF/LEF response element-driven luciferase reporter plasmid (#12456), and VEGF promoter-driven luciferase reporter plasmid (#66128) were acquired from Addgene (MA, United States). .. For transient transfection, 293T cells (2 × 105) were seeded in 6-well plates and incubated at 37°C for 24 h. Subsequently, each reporter plasmid was transfected using the Lipofectamine transfection reagent (#18324,012; Invitrogen; Thermo Fisher Scientific).

    Luciferase:

    Article Title: In vitro hair growth-promoting effects of araliadiol via the p38/PPAR-γ signaling pathway in human hair follicle stem cells and dermal papilla cells.
    Article Snippet: .. The pSV-βgalactosidase plasmid (#E1081) was sourced from Promega, whereas the Gli response element-driven luciferase reporter plasmid (#113712), TCF/LEF response element-driven luciferase reporter plasmid (#12456), and VEGF promoter-driven luciferase reporter plasmid (#66128) were acquired from Addgene (MA, United States). .. For transient transfection, 293T cells (2 × 105) were seeded in 6-well plates and incubated at 37°C for 24 h. Subsequently, each reporter plasmid was transfected using the Lipofectamine transfection reagent (#18324,012; Invitrogen; Thermo Fisher Scientific).



    Similar Products

    vegf promoter driven luciferase reporter plasmid  (Addgene inc)
    93
    Addgene inc vegf promoter driven luciferase reporter plasmid
    FIGURE 5 Araliadiol enhances hair growth-promoting signals and anagen phase markers in human hair follicle cells. (A) HHFSCs were treated with different concentrations of araliadiol (0–1.2 μg/mL) for 48 h. Protein levels of hair follicle stem cell-specific markers (K15 and K19), anagen phase markers (CD34 and p63α), and hair growth-promoting proteins <t>(VEGF</t> and ANGPTL4) were analyzed by Western blotting, with β-Actin serving as a loading control. (B) HDPCs were stimulated with indicated concentrations of araliadiol (0–2.5 μg/mL) for 48 h. Protein levels of hair growth-promoting proteins (FGF2, FGF7, FGF10, VEGF, IGF-1, and NOG) were analyzed by Western blotting, with β-Actin serving as a loading control. The protein levels in all blots were quantified using ImageJ software version 1.53t. K15, cytokeratin 15; K19, cytokeratin 19; CD34, cluster of differentiation 34; p63α, tumor protein 63 alpha; VEGF, vascular endothelial growth factor; ANGPTL4, angiopoietin like 4; FGF2, fibroblast growth factor 2; FGF7, fibroblast growth factor 7; FGF10, fibroblast growth factor 10; IGF-1, insulin-like growth factor 1; NOG, noggin.
    Vegf Promoter Driven Luciferase Reporter Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vegf+promoter+driven+luciferase+reporter+plasmid/pGL4%2E10-VEGFprom(-1000+to+-1)+(Plasmid+%2366128)/pm39691387-101-25-34
    Average 93 stars, based on 1 article reviews
    vegf promoter driven luciferase reporter plasmid - by Bioz Stars, 2026-11
    93/100 stars
    		  Buy from Supplier
    luciferase reporter plasmids driven by of the human vegf promoter  (Promega)
    90
    Promega luciferase reporter plasmids driven by of the human vegf promoter
    FIGURE 5 Araliadiol enhances hair growth-promoting signals and anagen phase markers in human hair follicle cells. (A) HHFSCs were treated with different concentrations of araliadiol (0–1.2 μg/mL) for 48 h. Protein levels of hair follicle stem cell-specific markers (K15 and K19), anagen phase markers (CD34 and p63α), and hair growth-promoting proteins <t>(VEGF</t> and ANGPTL4) were analyzed by Western blotting, with β-Actin serving as a loading control. (B) HDPCs were stimulated with indicated concentrations of araliadiol (0–2.5 μg/mL) for 48 h. Protein levels of hair growth-promoting proteins (FGF2, FGF7, FGF10, VEGF, IGF-1, and NOG) were analyzed by Western blotting, with β-Actin serving as a loading control. The protein levels in all blots were quantified using ImageJ software version 1.53t. K15, cytokeratin 15; K19, cytokeratin 19; CD34, cluster of differentiation 34; p63α, tumor protein 63 alpha; VEGF, vascular endothelial growth factor; ANGPTL4, angiopoietin like 4; FGF2, fibroblast growth factor 2; FGF7, fibroblast growth factor 7; FGF10, fibroblast growth factor 10; IGF-1, insulin-like growth factor 1; NOG, noggin.
    Luciferase Reporter Plasmids Driven By Of The Human Vegf Promoter, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vegf+promoter+driven+luciferase+reporter+plasmid/luciferase+reporter+plasmids+driven+by+of+the+human+vegf+promoter/pm21750048-95-16-40
    Average 90 stars, based on 1 article reviews
    luciferase reporter plasmids driven by of the human vegf promoter - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    FIGURE 5 Araliadiol enhances hair growth-promoting signals and anagen phase markers in human hair follicle cells. (A) HHFSCs were treated with different concentrations of araliadiol (0–1.2 μg/mL) for 48 h. Protein levels of hair follicle stem cell-specific markers (K15 and K19), anagen phase markers (CD34 and p63α), and hair growth-promoting proteins (VEGF and ANGPTL4) were analyzed by Western blotting, with β-Actin serving as a loading control. (B) HDPCs were stimulated with indicated concentrations of araliadiol (0–2.5 μg/mL) for 48 h. Protein levels of hair growth-promoting proteins (FGF2, FGF7, FGF10, VEGF, IGF-1, and NOG) were analyzed by Western blotting, with β-Actin serving as a loading control. The protein levels in all blots were quantified using ImageJ software version 1.53t. K15, cytokeratin 15; K19, cytokeratin 19; CD34, cluster of differentiation 34; p63α, tumor protein 63 alpha; VEGF, vascular endothelial growth factor; ANGPTL4, angiopoietin like 4; FGF2, fibroblast growth factor 2; FGF7, fibroblast growth factor 7; FGF10, fibroblast growth factor 10; IGF-1, insulin-like growth factor 1; NOG, noggin.

    Journal: Frontiers in pharmacology

    Article Title: In vitro hair growth-promoting effects of araliadiol via the p38/PPAR-γ signaling pathway in human hair follicle stem cells and dermal papilla cells.

    doi: 10.3389/fphar.2024.1482898

    Figure Lengend Snippet: FIGURE 5 Araliadiol enhances hair growth-promoting signals and anagen phase markers in human hair follicle cells. (A) HHFSCs were treated with different concentrations of araliadiol (0–1.2 μg/mL) for 48 h. Protein levels of hair follicle stem cell-specific markers (K15 and K19), anagen phase markers (CD34 and p63α), and hair growth-promoting proteins (VEGF and ANGPTL4) were analyzed by Western blotting, with β-Actin serving as a loading control. (B) HDPCs were stimulated with indicated concentrations of araliadiol (0–2.5 μg/mL) for 48 h. Protein levels of hair growth-promoting proteins (FGF2, FGF7, FGF10, VEGF, IGF-1, and NOG) were analyzed by Western blotting, with β-Actin serving as a loading control. The protein levels in all blots were quantified using ImageJ software version 1.53t. K15, cytokeratin 15; K19, cytokeratin 19; CD34, cluster of differentiation 34; p63α, tumor protein 63 alpha; VEGF, vascular endothelial growth factor; ANGPTL4, angiopoietin like 4; FGF2, fibroblast growth factor 2; FGF7, fibroblast growth factor 7; FGF10, fibroblast growth factor 10; IGF-1, insulin-like growth factor 1; NOG, noggin.

    Article Snippet: The pSV-βgalactosidase plasmid (#E1081) was sourced from Promega, whereas the Gli response element-driven luciferase reporter plasmid (#113712), TCF/LEF response element-driven luciferase reporter plasmid (#12456), and VEGF promoter-driven luciferase reporter plasmid (#66128) were acquired from Addgene (MA, United States).

    Techniques: Western Blot, Control, Software

    FIGURE 8 Araliadiol elevates hair growth-promoting effects in human hair follicle cells by upregulating the PPAR-γ signaling pathway. (A) Protein levels of PPAR-γ and hair growth-inductive proteins (K15, VEGF, and ANGPTL4) were assessed after treatment with araliadiol (1.2 μg/mL) with or without GW9662 (20 μM) in HHFSCs for 48 h. (B) Protein levels of PPAR-γ and hair growth-inductive proteins (FGF7, VEGF, and NOG) were assessed after treatment with araliadiol (2.5 μg/mL) with or without GW9662 (20 μM) in HDPCs for 48 h. Protein levels in all blots were quantified using ImageJ software version 1.53t.

    Journal: Frontiers in pharmacology

    Article Title: In vitro hair growth-promoting effects of araliadiol via the p38/PPAR-γ signaling pathway in human hair follicle stem cells and dermal papilla cells.

    doi: 10.3389/fphar.2024.1482898

    Figure Lengend Snippet: FIGURE 8 Araliadiol elevates hair growth-promoting effects in human hair follicle cells by upregulating the PPAR-γ signaling pathway. (A) Protein levels of PPAR-γ and hair growth-inductive proteins (K15, VEGF, and ANGPTL4) were assessed after treatment with araliadiol (1.2 μg/mL) with or without GW9662 (20 μM) in HHFSCs for 48 h. (B) Protein levels of PPAR-γ and hair growth-inductive proteins (FGF7, VEGF, and NOG) were assessed after treatment with araliadiol (2.5 μg/mL) with or without GW9662 (20 μM) in HDPCs for 48 h. Protein levels in all blots were quantified using ImageJ software version 1.53t.

    Article Snippet: The pSV-βgalactosidase plasmid (#E1081) was sourced from Promega, whereas the Gli response element-driven luciferase reporter plasmid (#113712), TCF/LEF response element-driven luciferase reporter plasmid (#12456), and VEGF promoter-driven luciferase reporter plasmid (#66128) were acquired from Addgene (MA, United States).

    Techniques: Software

    FIGURE 10 Araliadiol enhances VEGF secretion via the p38/PPAR-γ signaling pathway in human hair follicle cells. (A, B) Human hair follicle cells were treated with araliadiol (0–2.5 μg/mL) for 48 h, either with or without SB202190 (20 μM) or GW9662 (20 μM). VEGF-A secretion levels were quantified using an ELISA on conditioned medium from the treated cells. Minoxidil (2 μg/mL) was included as a positive control. Results are presented as the mean ± SD of three independent experiments and analyzed using a one-way analysis of variance followed by Tukey’s test. ***p < 0.001 compared with the vehicle-treated group. ##p < 0.01; ###p < 0.001 compared with the araliadiol-treated group. ELISA, enzyme-linked immunosorbent assay.

    Journal: Frontiers in pharmacology

    Article Title: In vitro hair growth-promoting effects of araliadiol via the p38/PPAR-γ signaling pathway in human hair follicle stem cells and dermal papilla cells.

    doi: 10.3389/fphar.2024.1482898

    Figure Lengend Snippet: FIGURE 10 Araliadiol enhances VEGF secretion via the p38/PPAR-γ signaling pathway in human hair follicle cells. (A, B) Human hair follicle cells were treated with araliadiol (0–2.5 μg/mL) for 48 h, either with or without SB202190 (20 μM) or GW9662 (20 μM). VEGF-A secretion levels were quantified using an ELISA on conditioned medium from the treated cells. Minoxidil (2 μg/mL) was included as a positive control. Results are presented as the mean ± SD of three independent experiments and analyzed using a one-way analysis of variance followed by Tukey’s test. ***p < 0.001 compared with the vehicle-treated group. ##p < 0.01; ###p < 0.001 compared with the araliadiol-treated group. ELISA, enzyme-linked immunosorbent assay.

    Article Snippet: The pSV-βgalactosidase plasmid (#E1081) was sourced from Promega, whereas the Gli response element-driven luciferase reporter plasmid (#113712), TCF/LEF response element-driven luciferase reporter plasmid (#12456), and VEGF promoter-driven luciferase reporter plasmid (#66128) were acquired from Addgene (MA, United States).

    Techniques: Enzyme-linked Immunosorbent Assay, Positive Control